Table 1.
Crystallographic statistics
| Crystal I | Crystal II | Crystal III | Crystal IV | |
|---|---|---|---|---|
| Data collection | ||||
| Space group | P212121 | P212121 | P212121 | P212121 |
| Cell dimensions | ||||
| a, b, c (Å) | 39.8, 49.5, 186.3 | 39.9, 49.4, 188.4 | 37.9, 47.2, 173.9 | 53.0, 60.6, 203.4 |
| α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 |
| Resolution (Å) | 93.1–2.50 (2.60 – 2.50)* | 50.0–2.88 (3.04 – 2.88) | 47.2–3.08 (3.25 – 3.08) | 50.0–4.80 (4.88 – 4.80) |
| Rmerge | 0.075 (0.504) | 0.054 (0.530) | 0.095 (0.260) | 0.113 (0.386) |
| <I>/<σI)> | 19.0 (0.4) | 23.9 (2.8) | 14.8 (2.5) | 21.6 (3.0) |
| Completeness (%) | 99.0 (92.9) | 99.6 (99.5) | 98.0 (97.1) | 89.6 (89.7) |
| Redundancy | 11.9 (6.2) | 5.1 (5.2) | 6.7 (3.9) | 8.0 (5.4) |
| Refinement | ||||
| Resolution (Å) | 50.0 – 2.80 (2.87 – 2.80) | 50.0 – 2.88 (2.96 – 2.88) | ||
| No. reflections | 9193 (658) | 8415 (580) | ||
| Rwork / Rfree | 20.6 (44.7) / 23.9 (51.5) | 21.5 (46.8) / 25.7 (56.2) | ||
| No. atoms | ||||
| RNA | 1932 | 1922 | ||
| Chromophore | 18 | 18 | ||
| Ligand/ion† | 103 | 27 | ||
| Water | 15 | 5 | ||
| B - factors | ||||
| RNA | 90.7 | 117.4 | ||
| Chromophore | 59.6 | 73.4 | ||
| Ligand/ion† | 110.5 | 128.9 | ||
| Water | 71.0 | 99.1 | ||
| r.m.s deviations | ||||
| Bond lengths (A) | 0.007 | 0.006 | ||
| Bond angles (°) | 1.80 | 1.80 | ||
*
Values in parentheses are for highest-resolution shell. One crystal was used for each data set.
†
Ligand/ion does not include the chromophore.