Figure 4.
Brightfield photomicrographs showing contralateral projections to the mid-level mouse suprachiasmatic nucleus (SCN) and adjacent hypothalamus. (A) Projections to the SCN are very dense (density = +++++), with moderately dense projections extending dorsally into the subparaventricular hypothalamus (sPa; density = ++) and medial anterior hypothalamus (AH; density = +; asterisk). Dorsolaterally, very sparse projections are found in close proximity to the fornix (arrow; cf. Fig. 1E–G). Very sparse innervation (density = ±) is present in the periventricular hypothalamus (arrowhead). (B) In a plane of section caudal to the SCN, sparse innervation is visible in the retrochiasmatic area (RCh; density = +), medial AH and sPa, with very sparse innervation (density = ±) present in the Pa (arrowhead). In this image, moderately dense innervation is seen in the peri-supraoptic nucleus (pSON; density = +++) and modestly dense innervation in the lateral hypothalamus (LH; density = ++). Bars = 200 μm.