Figure 2.
Surface plasmon resonance analysis of wild-type and mutant FGFR2c-FGF interactions. Sensorgrams of representative analyte injections of wild-type, S252W, P253R, D321A and S252L/A315S FGFR2c binding to (A) FGF2 (at 12.5 nM), (B) FGF4 (at 6.25 nM), (C) FGF5 (at 200 nM), (D) FGF10 (at 400 nM), (E) FGF16 (at 200 nM), (F) FGF18 (at 400 nM), (G) FGF19 (at 400 nM), and (H) FGF23 (at 400 nM). Analyte injections are colored as follows: wild-type FGFR2c in black, S252W FGFR2c in blue, P253R FGFR2c in green, D321A FGFR2c in yellow and S252L/A315S in red. The biosensor chip response is indicated on the y-axis (ΔRU) as a function of time (x-axis) at 25°C. Kinetic data are summarized in Table 1.