Figure 5. IL-2-rAAV treatment attenuates tissue damage of kidney.
(A) PAS staining of renal cortex (10× magnification) Mononuclear cell infiltration into cortical tubulointerstitial and perivascular areas is observed (arrows). (B) Renal histopathological assessment was shown in glomelular, tubulointerstitial and perivascular lesion (*p<0.05; mean ± SEM; n=6). (C) Urine albumin and creatinine concentration were measured by ELISA. Albumin-creatinine ratio of control-(white bars) and IL-2-(black bars )-rAAV-treated mice is shown at the indicated days after rAAV injection (two-way ANOVA, *p<0.05; mean ± SEM; n=6). (D) Absolute number of each fraction of leukocytes in kidneys was counted. CD45+ cell number from the kidney of MRL/MpJ mice (MpJ CD45+) is shown as a control (37). (E) Relative mRNA expression of indicated cytokines and chemokines in kidneys was detected by quantitative real-time PCR (*p<0.05, **p<0.01; mean ± SEM; n=6). Data were shown in control-rAAV-treated mice (white bars) and IL-2-rAAV-treated mice (black bars). Results are representative of three independent experiments.