Figure 7. A large fraction of MYC positive mature GC B cells are in cell cycle, and these cells are essential for GC maintenance.
(a) Real-time PCR analysis of Ccdn2 and Ccdn3 mRNA, of flow sorted FO and of day 10 MYCEGFP negative (D10N) and positive (D10P) GC B cells. Data are presented relative to Hprt and normalized to FO B cells, (mean and s.e.m. of triplicates). (b) Representative analysis by flow cytometry of the DNA of non-GC B cells and of D10N and D10P GC B cells. (c) Graphical representation of data presented in (b), (mean and s.e.m.) (d) Flow cytometry analysis of splenic GC B cells 13 days after SRBC immunization in mice of the indicated genotypes. Right panel analysis of reporter (hCD2) positive GC B cells. Data are representative of 5 (a), 3 (b, c) and 2 (d) independent experiments.