Figure 5.
S100A9 as a marker of CD11b+CD14+ myeloid-derived suppressor cells (MDSCs). (A) Representative histograms of flow cytometry analysis of S100A8 and S100A9 expression in CD11b+CD14+ cells from a patient with non–small cell lung cancer (NSCLC) (CA) and normal subject (NC). Gray lines, IgG control; black lines, S100A8 or S100A9 staining. (B) Column bar graph analysis of S100A8 and S100A9 expression determined by fluorescence-activated cell sorter scan. The data are the mean ± SEM and are presented as the percentage of positively stained cells (left panel) and mean fluorescence intensity (MFI, right panel). Open bars, normal CD11b+CD14+ cells; solid bars, NSCLC CD11b+CD14+ cells; n ≥ 4; *P < 0.05. (C) Correlation of S100A9+ cells in CD11b+CD14+ cells with the T-cell suppression ability of CD11b+CD14+ cells from patients with NSCLC. n = 8; Spearman r = −0.85; P = 0.01. (D) Correlation of the MFI of S100A9 to T-cell suppression ability from patients with NSCLC. Spearman r = −0.89; n = 6; *P = 0.033.