Skip to main content
. Author manuscript; available in PMC: 2014 Aug 12.
Published in final edited form as: J Biol Chem. 2005 Jan 10;280(13):12593–12601. doi: 10.1074/jbc.M410982200

Table I. Cell cycle distribution of MCF-7/DN-Erk and MCF-7/PD98039 cells.

The control MCF-7, MCF-7/DN-Erk, and MCF-7 cells treated with PD98039 or antisense GADD45β were treated with (+) or without (−) a single dose of 5-Gy radiation. Cells were collected 48 h after radiation and fixed in 85% ethanol and stained with propidium iodide (PI). PI-stained nuclear DNA content, indicating the distribution of cells in G0/G1, S, and G2, was determined with FACScan plus software (BD Biosciences), and the results of 1 × 104 counted cells were analyzed using Multicycle software.

Cell line 5 Gy G0/G1 S G2/M
MCF-7 − 69.15 27.76 3.09a
MCF-7 + 70.79 18.95 10.26a
MCF-7/DN-Erk − 65.07 25.26 9.67
MCF-7/DN-Erk + 65.24 20.53 14.23
MCF-7/PD98039 − 69.42 25.50 5.94
MCF-7/PD98039 + 71.19 23.03 7.59
MCF-7/anti-GADD − 67.22 23.50 8.77
MCF-7/anti-GADD + 69.39 19.44 11.11
a

p < 0.01 compared to sham-IR control cells.