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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Nat Chem Biol. 2014 Jun 15;10(8):626–628. doi: 10.1038/nchembio.1551

Figure 1. Characterization of ispinesib resistant clones.

Figure 1

(a) Structure of kinesin-5 inhibitor ispinesib. (b) Comparative analysis of twelve ispinesib-resistant clones (I1 – I12) against five MDR substrates: irinotecan (I), mitoxantrone (M), nocodazole (N), paclitaxel (P) and vinblastine (V). White box: similar activity as parental cells, Yellow box: moderate reduction in sensitivity, Red box: substantial reduction in sensitivity. Representative dose response curves and LD50 values are shown in Supplementary Fig. 2 and Supplementary Table 2. (c – d): Analysis of mitotic spindles in parental and ispinesib-resistant cells. Maximum intensity projections of DNA (blue) and tubulin (green), and an overlay of the two images are shown. (c,d) Parental HCT116 cells treated with ispinesib (50 nM, 4hrs) (c) or vehicle control (d) were fixed and processed for immunofluorescence. (e) Ispinesib-resistant Clone I7 treated with ispinesib (50 nM, 4hrs) was fixed and processed for immunofluorescence. Scale bar: 5 μm.