Figure 4. Comparison of the extent of TCRβ gene encoding of the gB-8p-specific Vβ10+ TCRβ repertoires involved in the CD8+ T cell responses to VACV-gB infection in mice of different ages.
Shown are the percentages of unique TCRβ clonotypes pooled across all mice per age group that require a particular minimal number of nucleotide (n.t.) additions (A) and the percentage of unique TCRβ clonotypes per mouse requiring no nucleotide additions (B), with the median values per age group represented by the horizontal line. Significant differences in the percentages of unique TCRβ clonotypes per mouse requiring no nucleotide additions were found across age groups (B; p=0.0007; Kruskal-Wallis). In particular, the percentage of unique TCRβ clonotypes per mouse requiring no nucleotide additions differed significantly between 7 days old mice and mice aged 2-3 months, 18 months and 22 months old. A Mann-Whitney test was used for each pairwise comparison, with the statistical significance for each pairwise comparison determined at p<0.0083 (*), using Bonferroni correction for multiple pairwise comparisons.