Fig. 4.
Effects of isoprenaline, isoprenaline plus carbachol, and verapamil in rat, mouse, and human EHTs. A: isoprenaline (100 nM)- and carbachol (10 μM)-induced changes in relative force. B: effect of verapamil (100–1,000 nM) on relative force. A and B, expressed as percentage of maximal peak force of each EHT of rat (n = 7 to 8), mouse (n = 4–8), and human (n = 4–8). Contraction time T1 (C) and relaxation time T2 (D) in the presence of isoprenaline (100 nM) and isoprenaline plus carbachol (10 μM) are shown. Kinetics were measured at 20% of the peak height. Data are means ± SE. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. basal condition (1-way ANOVA plus Dunnett post tests); ##P < 0.01 and ###P < 0.001 vs. rat (2-way ANOVA plus Bonferroni post tests).