Figure 2. Isolated CSCs display typical CSC characteristics.
(A) Intrinsic ABCG2 protein expression in control BC and SWCNT-exposed BSW cells was determined by Western blotting. (B) CSCs and their non-CSC counterpart were isolated from BSW cells based on their SP phenotype using FACS and designated as SP and NSP cells, respectively. Fluorescence analysis of Hoechst 33342 blue uptake in SP and NSP cells using fluorescence microscopy (left). Arrows indicate the ring-shaped staining pattern in SP cells. Quantitative analysis of the Hoechst blue fluorescence is shown (right). (C, D) Analysis of tumor sphere (C) and soft agar colony formation (D) in SP and NSP cells after two weeks of culture.