Skip to main content
. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Cell. 2014 Jun 19;157(7):1605–1618. doi: 10.1016/j.cell.2014.04.033

Figure 1. ECs Express and Secrete an Anti-angiogenic Isoform of VEGF-A.

Figure 1

(A) Neutralizing anti-VEGF-A antibody enhances EC migration. Shown are ECs migrating across razor-wound line (mean ± SE, n=3) (left), individual cell trajectories and root-mean-square displacement (center), and cell speed in the presence of anti-VEGF-A antibody (n=71) and IgG (n=52) (right).

(B) Neutralizing anti-VEGF-A antibody increases EC proliferation. Cell amount was determined fluorimetrically as DNA (mean ± SE, n=4).

(C) EC-derived conditioned medium inhibits EC migration. EC migration determined by razor-wound assay (mean ± SE, n=3).

(D) Conservation of VEGFA 3′UTR proximal region (gray); canonical and downstream, in-frame stop codons in mammals (boxed).

(E) Schematic showing generation of VEGF-A, VEGF-Ab, and VEGF-Ax isoforms.

See also Figure S1.