Figure 1. Human glioma cells produce IL-1 mRNA and proteins in response to IL-1 stimulation.
The production of IL-1β protein (intracellular A and secreted B) was examined in U251, U87, human fetal astrocytes and microglia (from left to right) in response to IL-1α, IL-1α/IFNγ, poly IC (PIC) and LPS. The GBM cell lines (U87 and U251) produced IL-1β in response to IL-1α with IFNγ acting as a primer. Little or no IL-1 was induced by poly IC and none by LPS. Microglial IL-1 protein was induced by LPS >poly IC >> IL-1 (± IFNγ). Human astrocytes did not produce IL-1β protein. (C) By real-time PCR, all four cultures including human astrocytes expressed IL-1β mRNA in response to IL-1/IFNγ. Data shown are mean ± SD (n = 3). ***p<0.001, **p<0.01, *p<0.05, n.s. = not significant compared to control (Ctr). Also see Table S1.