Figure 3.
QPCR gene expression changes are shown relative to control expression. A) 23 genes found to be significantly different using RNA-Seq (adj. p<0.05 or FoldChange >25%) were chosen to be further tested by QPCR. Genes marked with “*” were found to by QPCR to be significantly differentially expressed (p<0.05, t-test, N=10, FDR<10%) Symbols ("+" or "−") below each gene indicate whether the gene exhibits: 1) DESeq significance (adj. p<0.05) 2) DESeq FoldChange >25% 3) Evidence for involvement in neuropsychiatric disease(NPD). B) 10 genes not found to be differentially expressed by RNA-Seq, but thought to be relevant to psychiatric diseases were tested by QPCR, and results are shown relative to control expression. Genes marked with “*” were found to by QPCR to be significantly differentially expressed (p<0.05, t-test, N=10, FDR<10%). C) QPCR and RNA-Seq Correlation shown above. All 33 genes tested by QPCR are shown; open circles represent genes differentially expressed by RNA-Seq, and triangles represent genes not differentially expressed by RNA-Seq. The horizontal and vertical position of each dot represents QPCR Fold Change and RNA-Seq Fold Change, respectively. Pearson’s correlation coefficient was calculated after linear regression, R2=.53 (p<0.001). Best Fit slope = 1.02 +/− 0.17.