Figure 2. Matrix regulates VCAM-1 expression through NF-KB.
(A) HAECs plated on BM or FN were treated with oxLDL (100 μg/mL) for the indicated times. Immunoblotting was performed for P-NF-κB (p65, Ser536) and GAPDH. Representative Western blots are shown, n=5. (B) HAECs on different matrices were treated with oxLDL for 1 hour and NF-κB nuclear translocation determined by immunocytochemistry. Representative images are shown, n=3. (C) HAECs were treated with Bay11-7082 (10 μM, 1 hr) and oxLDL-induced VCAM-1 expression analyzed. Representative Western blots are shown, n=3. (D). oxLDL-induced VCAM-1 expression was determined in HAECs infected with either a CMV or SR-IκBα expressing adenovirus. Representative Western blots are shown, n=3. Values are means ± SE, *p < 0.05 compared with no treatment condition, #p < 0.05 comparing matrices.