Figure 7. Changes in fiber type patterns in intercostal muscle of end stage DM dogs.
Intercostal muscles were stained with anti-myosin heavy chain-1 to visualize the proportions of fiber types. (A) Unaffected PWC with normal checkerboard pattern of type 1 and 2 fibers. (B) Grade 4 PWC with a high predominance of type 1 fibers. Bar in (A) represents magnification in both images. (C) Bar graph displaying % of type 1 myofibers in PWC (left) and Boxer (right) thoracic intercostal muscle. PWC graph correlates with observations, with a larger percentage of type 1 fibers in grade 4 dogs (control n = 1, affected n = 4). (Boxers: control n= 1, affected n =2).