Figure 2.
Measurement of anxiety-like behaviors in rats using light/dark box (LDB) shows increased anxiety levels in ethanol-withdrawn rats. TSA treatment prevents the withdrawal-induced anxiogenic effect. The data are represented as percentage of time spent in light and dark compartment of LDB. The general activities of the rats are measured by total ambulations during the exploration of LDB. Values are the mean ± SEM of 6–7 rats in each group. * Significantly different (p < 0.001; one-way ANOVA followed by post hoc analysis by Tukey’s test; percentage of time spent in the light and dark compartments [F4, 26=43.71, p<.001]) from all other groups (Control+ Vehicle, Ethanol+ Vehicle, Withdrawal+ TSA, Control+ TSA injected rats). # Groups significantly different (p<0.001) from each other.