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. Author manuscript; available in PMC: 2014 Jul 8.
Published in final edited form as: Virology. 2013 Feb 12;438(1):37–49. doi: 10.1016/j.virol.2013.01.010

Figure 10. CHIKV nsP1 suppresses BST-2 expression.

Figure 10

CHIKVsp, mCherry tagged plasmids expressing E1 or nsP1, and yfpBST-2 were transfected or cotransfected into 293T cells. Figure shows: (A and B) FACS analysis showing level of surface yfpBST-2 upon co-transfection of CHIKV and E1 (A) or CHIKV and nsP1 (B). The bars are mean fluorescence intensity (MFI) while the histograms (inset) represent percent expression; (C) confocal images depicting expression levels of yfpBST-2, mCherryE1, and overlay of yfpBST-2/mCherryE1; (D) confocal images showing expression levels of yfpBST-2, mCherrynsP1, and overlay of both; (E) BST-2 mRNA levels following transfection with different CHIKV constructs. PCR data is normalized to GAPDH and presented as fold change relative to BST-2 transfected cells. Error bars are standard deviation; * is significance with p value less than 0.05.White boxes highlight areas of co-localization. Figure represents experiments repeated severally with similar results.