Figure 5.
Treatment of temporal artery–SCID mouse chimeras with AR inhibitors increases the lipid peroxidation downstream product HNE. Temporal artery samples from patients were transplanted subcutaneously into SCID mice. Temporal artery–SCID mouse chimeras were injected with either Sorbinil (50 mg/kg/day) or Zopolrestat (50 mg/kg/day) intraperitoneally for 1 week or were sham-treated. The temporal artery grafts were explanted after treatment and stained with antibodies to AR as described in Figure 2. AR expression (red stain) was detected in sections from sham-treated (a) as well as from Sorbinil-treated (b) tissues. Original magnification: ×400. (c) Kidneys were harvested after completion of the treatment, and tissue extracts were adjusted to 0.2 mg protein. HNE in the renal tissue was measured by a lipid peroxidation assay. (d and e) Frozen sections were stained by immunofluorescence with an HNE adduct–specific antibody. The stained area was quantified by laser confocal microscopy, and the percentage of stained tissue in relation to the entire arterial circumference was calculated. SCID, severe combined immunodeficiency.