Fig. 2.
TGHQ mediates nonapoptotic cell death in HK-2 cells. (A) Flow cytometric analysis of necrotic and/or apoptotic cells was determined by Annexin-V/PI staining. Cells were exposed to cisplatin (50μM) for 14 h as a positive control or TGHQ (400μM) for various periods of time (1, 2, 4, and 6 h). (B) The statistical analysis from three independent biological experiments. Data represent the mean ± standard deviation (n ≥ 3). *p < 0.05 compared with control group. (C) Caspase 3 cleavage was not detected in cells treated with TGHQ. Cells were exposed to 50μM cisplatin for 14 h or various concentrations of TGHQ (200, 400, or 800μM) for various periods of time (1, 2, and 4 h). Western blot analysis was performed. The illustrated blot is typical of at least three independent experiments. (D) The densitometric and statistical analysis of the image data. Data represent the mean ± standard deviation (n ≥ 3). *p < 0.05 compared with control group.