Figure 8.
Determination of blood neutrophil counts in mice chimeric for P-selectin expression and in P-selectin–null and wild-type mice. Peripheral blood was collected from bone marrow–transplanted mice 4 weeks after engraftment and before induction of nephrotoxic nephritis, and the numbers of neutrophils were determined. P-selectin–deficient mice (Pt–/EC–, white bar, n = 7) had approximately twofold more circulating neutrophils than did wild-type (Pt+/EC+, black bar, n = 4), whereas mice with P-selectin expression only in endothelial cells (Pt–/EC+, cross-hatched bar, n = 10) or only in platelets (Pt+/EC–, hatched bar, n = 11) had levels of circulating neutrophils that were not significantly different than those in wild-type mice. *P < 0.05.