Figure 2. Confirmatory assays for selected inhibitory and enhancing ISGs.
a, b, Independent confirmatory assays against DNA and +ssRNA viruses (a) and –ssRNA viruses (b) were carried out using new lentivirus stocks. Data were normalized to a Fluc control, highlighted in a yellow box and by dotted line. Data are presented as box and whisker plots; whiskers extend to show the highest and lowest values. Data represent six technical replicates to control for intra-assay variability. Statistical significance was determined by one-way analysis of variance (ANOVA) (*P <0.05, **P <0.01, ***P <0.001; NS, not significant). The 50% inhibitory and 150% enhancing effects are denoted by red and green dotted lines, respectively. c, Comparative analysis showing the frequency with which confirmed inhibitory or enhancing ISGs targeted +ssRNA or –ssRNA viruses.