Figure 3.
Effect of MAPT methylation on gene expression. (A) Scatter plot of MAPT methylation versus MAPT expression levels in cerebellum samples of Brain1 cohort (N = 69). Effect of MAPT methylation on PD. (B) In vitro analysis of relative expression in unmethylated (white) versus methylated (black) luciferase reporter constructs in SK-N-F1 cell. Error bars indicate standard error of the mean from five independent experiments. (C) Scatter plot of methylation versus PD age of onset from Leukocyte cohort (N=358). (D) Analysis of relative MAPT methylation in control (white, N = 12) and PD (black, N = 28) patients in cerebellum (W = 185, p = 0.034), anterior cingulate cortex (W = 210.5, p = 0.151) and putamen (W = 384.5, p = 0.001) from Brain2 cohort. Error bars indicate standard error of the mean. * = p < 0.05, ** = p < 0.001.