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Immunoblot analysis of the digitonin-induced release of the mitochondrial intermembrane compartment marker protein AlF (with anti-ALF antibody), the mitochondrial matrix marker protein mHSP70 (with anti-mHSP70 antibody) and hBFIT2 (with anti-Xpress antibody) from mitochondria isolated from hBFIT2/pcDNA4 transfected HEK293T/17 cells.
