Fig. 2.
IL-6Rα mRNA is constitutively present in human PMNs and is not increased in response to activation with PAF. Freshly isolated human PMNs were lysed immediately (time 0) or after activation with 10 nM PAF in suspension for the times shown, and transcript levels were analyzed by real-time PCR using probes specific for IL-6Rα or GAPDH (data not shown). (A) PCR amplification products for IL-6Rα mRNA at baseline and after activation with PAF. The levels of IL-6Rα mRNA did not change when PMNs were incubated for the same time intervals in the absence of PAF (data not shown). (B) The relative change in IL-6Rα transcript abundance as analyzed by real-time PCR is shown compared with that present at time 0 by using template from the same PCR analysis in A. The level at time 0 was arbitrarily set as 1. (C) Fluorescence curves used in the real-time analysis of IL-6Rα transcript levels in B. This pattern of IL-6Rα mRNA expression in unstimulated and PAF-activated PMNs was seen in five additional experiments.