FIGURE 1.
PD-1 is expressed on prostate resident T cells and PD-L1 on the prostate stroma. Naive 2C T cells were transferred into TRP-SIY mice, followed by intranasal infection with WSN-SIY virus. Prostates were harvested day 13 and day 36 post transfer, and Thy1.1+2C TCR+ cells were analyzed for PD-1 (A), Tim3 (B), or LAG-3 (C) expression. Histograms show relative levels of cell surface expression on 2C T cells at day 13 (gray) or day 36 (black), compared with isotype control (filled gray line). (D) Prostates from TRP-SIY (black) and C57BL/6-SIY (gray) mice were harvested and digested into single-cell suspension and stained with PD-L1 or isotype (filled gray line) and analyzed by flow cytometry. Histograms show relative levels of PD-L1 expression on prostate cells. (E) Sectioned prostates of TRP-SIY and C57BL/6-SIY mice were stained for PD-L1, visualized with peroxidase staining (brown), and counterstained with eosin (red). Arrows indicate areas of positive staining. Scale bars, 100 µm.