Figure 5.
(3α,5α)-3-hydroxypregnan-20-one (3α,5α-THP) colocalizes with StAR in the mPFC, CA1 pyramidal cells of the hippocampus, and NAc, whereas 3α,5α-THP colocalization with TSPO is observed in mPFC and CA1 hippocampus, but is minimal in NAc. In the CA1 hippocampus and NAc, TSPO is also located in cells that are not 3α,5α-THP-labeled. (a) In the mPFC, 3α,5α-THP (green) colocalizes with StAR (red, top panel) and TSPO (red, bottom panel). (b) In the CA1 hippocampus, 3α,5α-THP (green) colocalizes with StAR (red, top panel) and TSPO (red, bottom panel). Note the presence of TSPO labeling in a cell that is not labeled for 3α,5α-THP (yellow arrow). (c) In the NAc, 3α,5α-THP (green) colocalizes with StAR (red, top panel), but colocalization with TSPO is rarely observed (red, bottom panel). Note the presence of TSPO labeling in cells that are not labeled for 3α,5α-THP (yellow arrows). CA1, Cornu Ammonis area 1; mPFC, medial prefrontal cortex; NAc, nucleus accumbens; StAR, steroidogenic acute regulator protein; TSPO translocator protein.