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. 2014 Mar 19;39(8):1978–1987. doi: 10.1038/npp.2014.46

Figure 2.

Figure 2

ADX does not alter the effect of ethanol (2 g/kg, i.p.) on (3α,5α)-3-hydroxypregnan-20-one (3α,5α-THP) immunoreactivity in the CA1 hippocampus and polymorphic cell layer of the DG. (a) Ethanol increases 3α,5α-THP immunoreactivity in the CA1 pyramidal cell layer after sham surgery and ADX, compared with respective saline controls. (b) The red box indicates the location (−3.14 mm relative to bregma) of representative photomicrographs within the hippocampus. (c) Representative photomicrographs ( × 10) of 3α,5α-THP immunoreactivity in the CA1 pyramidal cell layer of sham saline (n=8), sham ethanol (n=9), ADX saline (n=10), and ADX ethanol (n=11) animals. (d) Ethanol increases 3α,5α-THP immunoreactivity in the polymorphic cell layer of the DG following sham surgery or ADX, when compared with respective saline controls. (e) The red box indicates the location (−3.60 mm relative to bregma) of representative photomicrographs within the DG. (f) Representative photomicrographs ( × 10) of 3α,5α-THP immunoreactivity in the polymorphic cell layer of the DG (oval). Ethanol or saline were administered 60 min before tissue fixation and collection. Data are expressed as the mean positive pixels/mm2±SEM. * indicates the main effect of ethanol treatment, P<0.0001. ADX, adrenalectomy; CA1, Cornu Ammonis area 1; DG, dentate gyrus; Sham, sham adrenalectomy surgery.