Figure 2.
Isolation of Intestinal Organoids Independent of the LGR5-GFP Reporter System
Expression profiling of organoids from GFP-positive and nonsorted LGR5-GFP teratoma cells compared with hESCs and fibroblast-like cells. qRT-PCR for the indicated genes in hESCs, organoids derived from eGFP-positive cells (n = 3), and nonsorted (n = 3) cells of LGR5-GFPN-term, hESCs, and fibroblast-like cells (derived from hESCs as described previously [Hockemeyer et al., 2008], expressing telomerase from the AAVS1 locus). Relative expression levels were normalized to baseline expression of these genes in hESCs. Data are biological replicates of independent experiments; bars represent the SEM. See also Figure S2.