FIG. 4.
Comparative sensitivity of RT-LAMP and RT-PCR for detection of SARS-CoV. The amplification by RT-LAMP (A) showed ladder-like pattern, whereas the RT-PCR (B) showed 195-bp amplification. (A) Sensitivity of SARS RT-LAMP assay as monitored by real-time measurement of turbidity (LA-200; Teramecs). Shown from left to right are the curves of decreasing concentrations of virus from 10,000 to 0.00001 PFU. The detection limit for the assay was 0.01 PFU. (B) Sensitivity of RT-PCR for the detection of SARS-CoV as observed by agarose gel analysis with a detection limit of 1 PFU.