Fig. 2.
Effect of recombinant adiponectin treatment in alcohol-fed adiponectin KO mice. A: daily serum samples from alcohol-fed adiponectin KO mice implanted with adiponectin-delivering Alzet pumps were measured for adiponectin by ELISA. #P < 0.05 compared with day 1. *P < 0.05 compared with day 3. B: serum high-molecular-weight (HMW) adiponectin assessed by ELISA at harvest. Sal, saline. C: representative liver histological sections from alcohol-fed adiponectin KO mice implanted with either saline- or adiponectin-delivering pumps stained with hematoxylin and eosin (top) or oil red O (bottom), scale bar = 100 μm. D: quantitation of triglyceride levels in liver extracts. E: liver-to-body weight ratio. F: mRNA extracted from livers of treated animals was analyzed for IL-6, SOCS3, MCP-1, and TNF levels by qRT-PCR. G: ALT activities in serum. Data presented as means ± SE. *P < 0.05, ***P < 0.01.