Figure 4.
Characterization of mouse GFP+MSCs. (A) Isolated and individually expanded GFP+MSCs were analyzed by fluorescence-activated cell sorting (FACS) using antibodies for Sca-1, CD29, CD45, and CD11b. (B) The ability of MSCs to differentiate into fibroblast/myofibroblast was evaluated by the expression of α-SMA (Abcam) with DAPI for nuclei immune-staining after cells were treated with CRE (50μg/ml) for 72 hours.
