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. Author manuscript; available in PMC: 2014 Jul 25.
Published in final edited form as: Mol Cell. 2013 Jun 27;51(2):249–264. doi: 10.1016/j.molcel.2013.05.026

Figure 4. The E-site tRNA increases the stability of post-TCs.

Figure 4

(A–C) Toe-printing analysis of ribosomal complexes obtained by treating pre-TCs formed on MVHL-STOP, MVHC-STOP or MVKC-STOP mRNAs with combinations of eRF1, eRF3, puromycin, eEF2 and ABCE1 in the presence/absence of tRNALys at the indicated free [Mg2+]. Black arrows show the positions of pre- and post-TCs. Red arrows indicate the positions migrated post-termination ribosomes. Upstream codons that are identical to the last codon of the encoded tetrapeptide are shown in red.