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. Author manuscript; available in PMC: 2014 May 26.
Published in final edited form as: Nucl Med Biol. 2010 Oct 27;38(2):223–233. doi: 10.1016/j.nucmedbio.2010.08.013

Fig. 4. Metal specificity of improved mutant.

Fig. 4

Competition equilibrium isotherms for C8.2.5 scFv displayed on the surface of yeast for binding to DOTA-metal (A) and DOTA-Bn-metal (B) complexes measured with a competition-based assay. It should be noted that 100 pM DOTA-Bnbiotin-Y is the detected label. The binding constant for the competitor will follow a Cheng-Prusoff relationship as described in Materials & Methods.