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. Author manuscript; available in PMC: 2015 Mar 12.
Published in final edited form as: Cell Host Microbe. 2014 Mar 12;15(3):363–373. doi: 10.1016/j.chom.2014.02.004

Fig. 3. The HCMV miRNAs facilitate formation of the viral assembly compartment.

Fig. 3

(A) NHDFs were infected with either the AD169 wild type (WT) or the AD169 miR-US5-1, -US5-2, -UL112-1 triple mutant virus (Mut) or mock-infected (Mock) and analyzed by immunofluorescence with the markers indicated as described in the Materials and Methods. (B-E) HeLa cells were transfected with a combination of HCMV miRs US5-1, US5-2, and UL112-1 (B), combinations of pathway specific siRNAs SNAP23, RAB5C, RAB11A, and CDC42 (C) or SNAP23, RAB5C, and RAB11A (D), or non-targeting negative control siRNA (E) and the Golgi complex was evaluated by immunofluorescence 4 days post-transfection for the cis-Golgi marker GM130 in red and the trans-Golgi marker Golgin245 in green as described in the Materials and Methods. Figure 3, related to Figure S5.