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. 2014 Jun;20(6):867–881. doi: 10.1261/rna.044479.114

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© 2014 Egan et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 4. — The roles of NURS complex subunits in RNA elimination. (A,B) Quantitative RT-PCR analysis of meiotic mRNA (A) and centromeric dg transcript (B) levels in wild-type cells and cells carrying deletions of the genes encoding the nonessential subunits of NURS. (C) Western blot showing the levels of auxin-inducible degron (AID)-tagged proteins, some driven by the thiamine-repressible nmt promoter, after the addition of DMSO (−) or thiamine and the synthetic auxin NAA (+). The asterisk indicates a protein that cross-reacts with the α-HA antibody. Molecular weight markers (kDa) are shown on the right. (D,E) Quantitative RT-PCR analysis of meiotic mRNA (D) and centromeric dg transcript (E) levels in wild-type cells and in cells expressing AID-tagged proteins after the addition of DMSO (−) or thiamine and NAA (+). Error bars represent standard deviation (SD). wt, wild-type.