Figure 1.
A Raw data. Representative whole-cell current traces recorded at different temperatures from HEK293 cells stably expressing either WT (left) or R1448H (right) mutant channels: 10°C (top), 20°C (middle) and 30°C (bottom). Note the slowed inactivation of the mutant. B Temperature effect on amplitude. Temperature dependency of currents through WT or R1448H Nav1.4 channels normalized to values at 30°C. C Temperature effect on flux. Temperature dependency of Na+ influx through WT or R1448H Nav1.4 channels. For B and C, values are mean ± SEM (n = 6). SEM is shown as bars and * indicates a significant difference between WT and R1448H (p < 0:05). Note that while the amplitude shows similar temperature dependence, the flux of the mutant is increased due to the slowed inactivation.