FIG 1.
CVB3 and virus-CAR complex maps. (A) The close-up view of the asymmetric unit of CVB3 shows the topography of the virus surface, including the 5-fold mesa, canyon, and puff. The compass points are used to describe the details of the canyon and the canyon rim. (B) Surface-rendered cryo-EM reconstruction of CVB3-CAR complex formed at 4°C. The map is colored radially according to the key and displayed at a sigma value of 1. One asymmetric unit is outlined in black. The cutaway view shows the interior of the reconstruction with a fully packaged genome (red). (C) Central section of CVB3-CAR complex showing strong density for CAR and labeled 2-, 3-, and 5-fold symmetry axes. (D) Fourier shell correlation versus spatial frequency of the icosahedrally averaged reconstruction indicate resolution of the map where the Fourier shell correlation curve crosses the value of 0.5 (dashed red line). (E) The D1D2 CAR structure (blue) fitted into the difference density (blue mesh) is displayed onto a surface-rendered, radially colored CVB3 map to show CAR binding to the puff region and north canyon rim.