FIG 8.
NCX1 overexpression rescues histopathology of the diaphragm of Sgcd−/− mice. (A) Representative H&E-stained histological images (×40 magnification) from the diaphragms of Sgcd−/− and Sgcd−/− NCX1 TG mice at 6 weeks of age. (B) Percentages of fibers with centrally localized nuclei quantified from the diaphragm in Sgcd−/− and Sgcd−/− NCX1 TG mice. *, P < 0.05 versus Sgcd−/− mice. Numbers in the bars represent the number of mice analyzed. (C) Fibrotic areas (percent) from trichrome-stained histological sections of diaphragms at 6 weeks and 6 months in Sgcd−/− and Sgcd−/− NCX1 TG mice. (D) Images (×40 magnification) of von Kossa-stained histological sections of Sgcd−/− and Sgcd−/− NCX1 TG diaphragm muscle at 6 weeks of age. (E) TUNEL-positive myofibers in the diaphragms of WT, NCX1 TG, Sgcd−/−, and Sgcd−/− NCX1 TG mice at 6 weeks of age. *, P < 0.05 versus Sgcd−/− mice. (F) Central nucleation measured in Sgcd−/− and Sgcd−/− NCX1 TG-low mice in histological sections from diaphragm, gastrocnemius, and quadriceps. (G) Percent fibrosis measured in Masson's trichrome-stained histological sections from the indicated muscles of Sgcd−/− and Sgcd−/− NCX1 TG-low mice. *, P < 0.05 versus Sgcd−/− mice. The error bars indicate SEM.