FIG 1.
No evidence of OAS in H1N1-primed ferret T-cell response. An ELISpot assay was performed to measure the T-cell response against the priming virus (self) and CA/09 following a challenge infection with the CA/09 virus. Ferret PBMCs collected 28 days following a challenge infection with the CA/09 virus were stimulated with BPL-inactivated virus (500 HA units/ml) to promote ferret IFN-γ release. PMA plus ION was used as a control antigen. Spots were counted with an ImmunoSpot Analyzer. BPL-inactivated FW/50 and B/Mal viruses did not stimulate a T-cell response and are not included.