Figure 2.
General Views of the CrPV-IRES/80S Complexes and Ratcheting Movements
(A) Superposition of the canonical (yellow) and rotated (gray) states of the 40S subunit showing a counter-clockwise rotation.
(B) Conformation of the CrPV-IRES in the rotated and canonical states of the ribosome in (A).
(C) Interaction of the CrPV-IRES with the L1 stalk of the 60S subunit.
(D) The conformation of the L1 stalk in different states of the ribosome. The stalk conformation with CrPV-IRES is from this work, whereas those with E-site tRNA and a hybrid P/E tRNA are from bacterial crystal structures (Gao et al., 2009; Tourigny et al., 2013).
(E and F) Ribbon diagram (left) and schematic (right) showing the simultaneous interaction of CrPV-IRES with the L1 stalk in the 60S subunit and elements of the 40S subunit.