Fig. 2.
Sema3F expression in normal human retina. (A, C, E) In analogy to the results seen in murine eyes, Sema3F localizes to the outer retinal border at the photoreceptor/RPE interface in human retinas. Images show representative samples at three different magnifications. (B, D) Negative controls without primary antibody demonstrate only weak background fluorescence. Note the complete lack of the specific Sema3F hyperfluorescent band at the photoreceptor/RPE interface despite increased exposure time in negative control images. Some red blood cells trapped in choroidal capillaries display autofluorescence in the negative control images.