Figure 5.
Bromodeoxyuridine (BrdU)-labeled cells in the hippocampal dentate gyrus express neuronal and astroglial markers. (A) Immunodetection of BrdU and NeuN in the hippocampal dentate gyrus for each condition. (B) Coexpression of BrdU and the immature neuronal marker doublecortin (DCX) in the dentate gyrus of the hippocampus. (C) BrdU-labeled cells also express the astroglial marker glial fibrillary acidic protein (GFAP) in the hippocampal dentate gyrus. (D) Similar proportions of BrdU-labeled cells express neuronal or astroglial markers across groups. For the micrographs from middle cerebral artery occlusion (MCAO) mice in panels A–C, the contralateral hippocampus is shown. AL, ad libitum; IF, intermittent fasting.