Figure 3. Camk4–/– mice are resistant to EAE.
EAE was induced in WT and Camk4–/– mice by immunization with MOG35–55 emulsified in CFA. (A) The clinical score of EAE and (B) body weight in these mice were monitored (*P < 0.05; mean ± SEM; cumulative results of 3 independent experiments with 3 to 5 mice per group). (C) Flow cytometry of intracellular IL-17 and IFN-γ at day 8 in CD4+ T cells obtained from mononuclear cells isolated from the draining inguinal lymph nodes (dLNs) and spleens of WT and Camk4–/– mice immunized with MOG35–55 emulsified in CFA to induce EAE and then activated in vitro with PMA and ionomycin (**P < 0.01; mean ± SEM; n = 3–4). Data are representative of 2 independent experiments with 3 to 4 mice per group.