Figure 2.
ICG-001 downregulates survivin in ALL (LAX7R) cells and blocks occupancy of CBP at survivin promoter. (a) Real time PCR was applied to confirm downregulation of survivin gene expression by ICG-001 (10µM) on day 3 post-treatment in LAX7R cells. * p<0.05. (b) Western blot of survivin in ALL cells treated with DMSO (0.1%) or ICG-001 (10µM). β-Actin was used as protein loading control. (c) ChIP analysis of ICG-001 treated LAX7R cells in the presence or absence of ICG-001 for occupancy of CBP and p300 at survivin promoter. (d) Cell numbers were assessed by trypan blue exclusion of dead cells at 48 hours post-treatment of ALL cells (LAX7R) with either DMSO (0.1%) or ICG-001 at 10µM. *p<0.05 (e) Live cells (Annexin V−/7-AAD−) were measured by Annexin V and 7-AAD staining using flow cytometry.