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. Author manuscript; available in PMC: 2015 Jul 2.
Published in final edited form as: Oncogene. 2013 Dec 2;34(1):15–26. doi: 10.1038/onc.2013.519

Figure 2. Kinase client signalling activity following CDC37 overexpression.

Figure 2

(a) Western blot showing phosphorylated ERK1/2, ERK2, phosphorylated MEK1/2, MEK1/2, phosphorylated RB (S795 and 780) and CYCLIN D1 expression in wildtype (WT), empty vector-transfected (V) or CDC37-overexpressing HCT116 (C1, C10) and HT29 (C8, C12) cells. ERK1/2 phosphorylation is quantified by densitometry in Supplementary Figure S3. (b) CDK4 kinase assay in wildtype (WT), empty vector-transfected (V) or CDC37-overexpressing HCT116 (C1, C10) and HT29 (C8, C12) cells. Following CDK4 immunoprecipitation, and incubation with recombinant RB, S795-phosphorylated RB and hypophosphorylated RB were measured by western blotting.