Figure 4. Summary of clonal lineage and kinetic relationships over time.
(A) Pearson correlation coefficients comparing barcode contributions for all barcodes falling above the sampling threshold of 1144 for pairwise comparisons, between all lineages and time points, for animal ZH33 and ZG66 (r values and their associated p values and 95% confidence intervals are given in Table S5). The color scale bar for r values is on the right. Slope indicates negative or positive correlation; the shape and the color signify the strength of the correlation. MDC-myeloid dendritic cells, PDC-plasmacytoid dendritic cells, LN-lymph node. Alternative analysis utilizing Spearman correlation shown in Fig S6 and Table S6 (B) Fraction of highly biased (>10 fold greater fractional representation in one sample versus the other) barcodes between all lineages and time points for ZH33 and ZG66. Bias is towards the sample shown in the row, and away from the sample in the column. Color scale is given on the right. P values <0.05 are starred; the frequency of highly biased clones in these comparisons differed significantly from the total frequency of highly biased clones at the time point combination. Hierarchical clustering analysis for fractional barcode contributions in ZH33 and ZG66 given in Fig S7.