Figure 2. Barcode copy number per HSPC clone.
(A) Barcode copy number in individual CFU obtained at the end of transduction (left, n=48) or from marrow CFU post-transplant from ZH33 (right, n=14). (B) Barcode copy number per transduced cell for PB samples. The molecular marking level (vector copy number) was determined by qPCR on sorted GFP negative granulocytes (GFP− Gr), GFP positive granulocytes (GFP+ Gr) and sorted T, B, and NK cells from ZH33, ZH17, and ZG66, and plotted on the left panel (white bars) next to the fraction of GFP+ cells in the same sample as assessed by FACS (green bars). A ratio of 1 implies one vector copy per each GFP+ cell. On the right, the ratio of molecular marking level determined by copy number PCR/GFP marking level for all samples from each of the three animals (mean±SEM) are shown.