Table 1.
Frequency of B-cell subpopulations in bone marrow and spleen in Zfp318 point mutant mice and in the blood of Zfp318−/−
| Genotype | Organ | B cells | Pro-B cells | Pre-B cells | Immature B cells | Mature B cells | Transitional B cells | Follicular B cells | MZ B cells | No. of animals |
| WT | BM | 21.1 ± 2.7 | 9.0 ± 1.5 | 22.1 ± 0.6 | 15.1 ± 1.0 | 36.5 ± 0.4 | — | — | — | 5 |
| Het | BM | 20.4 ± 2.1 | 8.4 ± 0.9 | 18.7 ± 3.2 | 14.2 ± 0.9 | 39.6 ± 3.2 | — | — | — | 6 |
| Hom | BM | 21.1 ± 2.0 | 7.4 ± 2.4 | 17.2 ± 3.2 | 12.6 ± 2.5 | 45.2 ± 7.6 | — | — | — | 4 |
| WT | Spleen | 57.9 ± 3.9 | — | — | — | — | 25.5 ± 7.0 | 56.0 ± 4.7 | 10.9 ± 3.4 | 9 |
| Het | Spleen | 56.1 ± 4.6 | — | — | — | — | 25.4 ± 6.8 | 56.9 ± 4.7 | 10.3 ± 2.1 | 10 |
| Hom | Spleen | 51.0 ± 3.0 | — | — | — | — | 21.9 ± 7.2 | 59.6 ± 5.2 | 11.4 ± 2.8 | 7 |
| Zfp318+/+ | Blood | 62.0 ± 4.8 | — | — | 21.4 ± 5.1 | 71.7 ± 6.7 | — | — | — | 4 |
| Zfp318+/− | Blood | 57.9 ± 5.1 | — | — | 17.5 ± 4.2 | 74.6 ± 5.0 | — | — | — | 22 |
| Zfp318−/− | Blood | 53.5 ± 4.2 | — | — | 19.1 ± 6.1 | 69.8 ± 7.4 | — | — | — | 15 |
The frequencies are expressed as mean values ± SD out of all live cells (B cells) or as a percentage of all B cells for all other populations. The last column shows the number of animals analyzed per genotype analyzed in one (BM and blood) or two (spleen) experiments. Statistical analysis was done comparing all groups within one organ by one-way ANOVA followed by Bonferroni post hoc test to compare each population in the heterozygous and homozygous mice to the relevant WT control. Statistically significant differences are marked in bold. B cells, B220+ in live lymphocytes gate for BM and in lymphocyte gate for spleen. Immature B cells, IgM+CD93+; mature B cell, CD93−; pre-B cell, CD24hiCD43−CD93+IgD−IgM−; pro-B cell, CD24intCD43+CD93+IgD−IgM−; in the bone marrow. Follicular, CD93−CD23hiCD21med; marginal zone B cells, CD93−CD23−CD21hi; transitional B cells, CD93+; in the spleen and total B cells (CD19+), immature (CD93+), and mature (CD93−) B cells in the blood of Zfp318−/− mice as well as Zfp318+/− and Zfp318+/+ littermate controls.