Table 1.
Reported substrate specificities for enzymes that methylate H3K36
| Enzyme* | Assay conditions | Histone substrates identified | Refs |
|---|---|---|---|
| NSD1 (KMT3B) | Nucleosomes | H3, H3K36, H3K36me1, H3K36me2, H4K20 | 14, 16–18 |
| Cell based and/or in vivo | H3K36me1, H3K36me2, H4K20me3 | 17–19, 78 | |
| NSD2 (WHSC1, MMSET) | Nucleosomes | H3, H3K36, H3K36me1, H3K36me2 | 16,17, 19,30 |
| Cell based and/or in vivo | H3K27me2‡, H3K36me2 | 17,28, 36 | |
| NSD3 (WHSC1L1) | Nucleosomes | H3, H3K36 | 16 |
| Cell based and/or in vivo | H3K36me2 | 40 | |
| ASH1; ASH1L | Nucleosomes | H3, H3K36, H3K36me2, H3K36me3§ | 41,48, 49 |
| Cell based and/or in vivo | H3K4, H3K4me3 | 49 | |
| SMYD2 | Core histones (octamers) | H3, H3K4, H3K36me2 | 42,43 |
| SETMAR (METNASE) | Core histones (octamers) | H3K4, H3K36me2 | 12 |
| Cell based and/or in vivo | H3K36me2 | 96 | |
| SETD2 (KMT3A, HYPB) | Nucleosomes | H3, H3K36me1, H3K36me2, H3K36me3 | 34,64 |
| Cell based and/or in vivo | H3K36me3 | 25,33,34 | |
| SETD3 | Core histones (octamers) | H3K4, H3K36 | 11 |
Table entries for the knockdown (cell based) or knockout (in vivo) analysis indicate a reported decrease in a given methylation state. ASH1, Absent, small and homeotic discs 1; ASH1L, ASH1-like; H, histone; NSD, nuclear receptor SET domain-containing; me1, monomethylated; me2, dimethylated; me3, trimethylated; SETD, SET domain-containing; SETMAR, SET domain and mariner transposase fusion gene-containing; SMYD2, SET and MYND domain-containing 2.
*
Alternative names are provided in brackets.
‡
RE-IIBP isoform of NSD2.
§
Gln2265Ala mutant of ASH1L.